Lachat Method: Ammonia 12-107-06-1-B

Document ID

Document ID ME148

Published Date

Published Date 01/18/2022
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Method Number
Lachat Method: Ammonia 12-107-06-1-B
Summary
Method # Range MDL Units Matrix Rev. Date
12-107-06-1-B 1.0-20.0 0.035 mg/L NH3-N Soil Extracts 15-Sep-08
Principle
Exchangeable ammonium is extracted from soil with 2 M KCl. The KCl extract is filtered and the filtrate is analyzed for ammonia by the phenolate method. This method is based on the Berthelot reaction. Ammonia reacts with alkaline phenol, then with sodium hypochlorite to form indophenol blue. Sodium nitroprusside (nitroferricyanide) is added to enhance sensitivity. The absorbance of the reaction product is measured at 630 nm, and is directly proportional to the original ammonia concentration.

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