DR300_Colorimetry Claros Enabled App Note

Document ID

Document ID CO351

Published Date

Published Date 01/17/2019
DR300_Colorimetry Claros Enabled App Note
Spectrophotometry is an analytical technique used for measuring the concentration of an analyte dissolved in a solution. The measurement of the intensity of light transmitted by a solution may frequently be used for the quantitative determination of a substance dissolved in the solution, if the substance is itself colored (absorbs light) or can be converted to a colored compound. Such a method of analysis is known as a colorimetric method or a spectrophotometric method. The two terms are generally used interchangeably, although the earlier term colorimetry referred to methods using visible light and matching by eye. Spectrophotometry is now more generally used because analyses can be made using ultraviolet and infrared light as well as visible light and because measurements of intensity are now more commonly made using electronic detectors. Visible color is not essential for quantitative analysis, but the analyte must absorb some portion of the electromagnetic radiation passing through it, whether from the ultraviolet, visible, or infrared portion of the spectrum. Spectrophotometrric methods of analysis are invariably comparison methods, where the unknown is compared to a standard of the same analyte under identical conditions of chemistry and lighting.

The physical laws describing the absorption of light on passagethrough a transparent material are relatively simple and may be applied to determining the amount of some absorbing analyte dissolved in a transparent solvent. The nature of light as electromagnetic radiation will be discussed as background for an understanding of how a spectrophotometer works and the principles of wavelength selection for chemical analysis.

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