Question
Microbiology Guide: Positive and Negative Controls for Media
Summary
Microbiology Guide: Media and Equipment Selection; Positive and Negative Controls for Media
Answer
From:
Microbiology Guide: Media and Equipment Selection
- Postive and Negative controls are not available for purchase from Hach
- Company recommendation: Microbiologics
| Hach Media | Positive Control | Negative Control |
| A-1 Broth | E. coli | E. faecalis (No growth at 44.5 °C) |
| Azide Dextrose Broth | E. faecalis | E. coli or S. epidermis |
| Bile Esculin Azide Agar | E. faecalis or E. aerogenes | E. coli |
| Brilliant Green Bile Broth | E. faecalis or S. epidermis | |
| EC Broth | E. coli | E. aerogenes or E. faecalis |
| EC Broth w/ MUG | E. coli | E. aerogenes or E. faecalis |
| EMB Agar | E. coli or E. aerogenes | E. faecalis (noting partial ihibition) |
| KF Strep Agar | E. faecalis | E. coli or E. aerogenes |
| Lactose Broth | E. coli or E. aerogenes | S. epidermis (may grow but no gas) |
| Lauryl Tryptose Broth | E. coli or E. aerogenes | S. epidermis (may grow but no gas) |
| LT Broth w/ MUG | E. coli | S. epidermis (may grow but no gas) |
| m-ColiBlue24® Broth | E. coli or E. aerogenes | E. faecalis |
| m-E Agar | E. faecalis | E. coli |
| m-Endo | E. coli or E. aerogenes | S. epidermis |
| m-Endo LES Agar | E. coli or E. aerogenes | S. epidermis |
| m-FC | E. coli | E. faecalis |
| m-FC w/ Rosolic Acid | E. coli | E. faecalis |
| m-Green Y&M Broth and Paddle Tester (Y&M side only) | A. niger or S. cerevisiae | None |
| m-TEC | E. coli | None |
| m-TGE | E. coli or S. epidermis | None |
| m-TGE w/ TTC | E. coli or S. epidermis | None |
| Nutrient Agar and Nutrient Broth | E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, or S. epidermis | None |
| Nutrient Agar w/ MUG | E. coli | S. epidermis (may grow but no gas and not fluorescence) |
| P-A Broth | E. coli or E. aerogenes | S. epidermis |
| P-A w/ MUG | E. coli | S. epidermis |
| Paddle Tester (HAB side) | E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, or M. luteus | None |
| Paddle Tester (TC side) | E. coli or E. aerogenes | None |
| PathoScreen™ Media | C. feundi | None |
| Plate Count Agar | E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus | None |
| R2A Agar | E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus | None |
| Total Bacteria Tubes | E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus | None |
| Tryptic Soy Agar and Broth | E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus | None |
| Violet Red Bile Agar | E. coli | S. epidermis |