Microbiology Guide: Positive and Negative Controls for Media

Document ID

Document ID TE8863

Version

Version 3.0

Status

Status Published

Published Date

Published Date 09/06/2018
Question
Microbiology Guide: Positive and Negative Controls for Media
Summary
Microbiology Guide: Media and Equipment Selection; Positive and Negative Controls for Media
Answer
From: Microbiology Guide: Media and Equipment Selection
  • Positive and Negative controls are not available for purchase from Hach
    • Company recommendation: Microbiologics
Positive and Negative controls for Hach biological growth media
Hach Media Positive Control Negative Control
A-1 Broth E. coli E. faecalis (No growth at 44.5 °C)
Azide Dextrose Broth E. faecalis E. coli or S. epidermis
Bile Esculin Azide Agar E. faecalis or E. aerogenes E. coli
Brilliant Green Bile Broth   E. faecalis or S. epidermis
EC Broth E. coli E. aerogenes or E. faecalis
EC Broth w/ MUG E. coli E. aerogenes or E. faecalis
EMB Agar E. coli or E. aerogenes E. faecalis (noting partial inhibition)
KF Strep Agar E. faecalis E. coli or E. aerogenes
Lactose Broth E. coli or E. aerogenes S. epidermis (may grow but no gas)
Lauryl Tryptose Broth E. coli or E. aerogenes S. epidermis (may grow but no gas)
LT Broth w/ MUG E. coli S. epidermis (may grow but no gas)
m-ColiBlue24® Broth E. coli or E. aerogenes E. faecalis
m-E Agar E. faecalis E. coli
m-Endo E. coli or E. aerogenes S. epidermis
m-Endo LES Agar E. coli or E. aerogenes S. epidermis
m-FC E. coli E. faecalis
m-FC w/ Rosolic Acid E. coli E. faecalis
m-Green Y&M Broth and Paddle Tester (Y&M side only) A. niger or S. cerevisiae None
m-TEC E. coli None
m-TGE E. coli or S. epidermis None
m-TGE w/ TTC E. coli or S. epidermis None
Nutrient Agar and Nutrient Broth E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, or S. epidermis None
Nutrient Agar w/ MUG E. coli S. epidermis (may grow but no gas and not fluorescence)
P-A Broth E. coli or E. aerogenes S. epidermis
P-A w/ MUG E. coli S. epidermis
Paddle Tester (HAB side) E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, or M. luteus None
Paddle Tester (TC side) E. coli or E. aerogenes None
PathoScreen™ Media C. feundi None
Plate Count Agar E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus None
R2A Agar E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus None
Total Bacteria Tubes E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus None
Tryptic Soy Agar and Broth E. coli, F. Aerogenes, E. faecalis, P. aeroginosa, S. epidermis, M. luteus None
Violet Red Bile Agar E. coli S. epidermis

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