Microbiology Guide: Other Microbiology Media (BART™, Paddle Testers)

Document ID

Document ID TE8862

Published Date

Published Date 01/11/2022
Microbiology Guide: Other Microbiology Media (BART™, Paddle Testers)
Microbiology Guide: Media and Equipment Selection; Other Micro Biology Media (BART™, Paddle Testers)
From: Microbiology Guide: Media and Equipment Selection
  • Biological Activity Reaction Test (BART™)
    • Media Packaging Options and Description
      • BART™ Test for Acid Producing Bacteria (APB), pk/9 (Product # 2831409) Method DOC316.53.01328
        • Bacterial strains capable of acid production under reductive (oxygen deprived) conditions
      • BART™ Test for Blue-Green Algae, pk/9 (Product # 2432709), pk/27 (Product # 2432727) Method LIT8436 (Multiple test methods in document)
        • Photosynthetic plant-like microorganisms: cholrophyceae, cyanobacteria, desmids, diatoms and euglenoids
      • BART™ Test for Denitrifying (DN) Bacteria, pk/9 (Product # 2619309) Method LIT8436 (Multiple test methods in document)
        • Nitrate reducing bacterial strains
        • Measures denitrifying bacteria which are able to reduce nitrate to nitrogen gas.
        • This test is applicable to any waters where there is likely to be potential septic or organic contamination. The presence of denitrifiers would indicate a potential health risk due to either septic wastes or nitrates in the water.
        • Gas forms a foam of bubbles around the ball, usually within three days. The presence of this foam by the end of day two is taken to be an indication of an aggressive population of denitrifying bacteria.
        • Absence of foam, regardless of any clouding of the water, indicates that the test is negative for the detection of denitrifying bacteria.
      • BART™ Test for Fluorescing (FLOR) Pseudomonas, pk/9 (Product # 2432609) Method LIT8436 (Multiple test methods in document)
        • Fluorescing pseudomonad strains and related bacteria
        • Pseudomonads are bacteria that are very well adapted to breaking down some chemicals such as jet fuel and solvents, but also can infest recreational waters and cause conditions ranging from skin, eye, ear, and nose infections to pneumonia-like infections.
        • The infectious pseudomonads produce an ultraviolet fluorescence that is usually a pale blue color.
        • Presence for this test means that either a greenish-yellow or a pale blue glow is generated by the careful application of an ultraviolet light just below the ball. The degraders tend to generate the greenish-yellow glow while the health risk group generates the pale blue glow.
        • A negative indication occurs when the sample remains clear.
      • BART™ Test for Heterotrophic Aerobic Bacteria (HAB), pk/9 (Product # 2490409), pk/27 (Product # 2490427) Method DOC316.53.01329
        • Broad spectrum of aerobic bacteria
        • Determines the amount of activity of heterotrophic aerobic bacteria; the most common application is wastewater.
        • This test relies upon the ability of the heterotrophic aerobic bacteria to reduce a methylene blue dye. To add the methylene blue to the sample, the test vial once charged is simply placed upside down for 30 seconds to allow the blue color to develop.
        • A positive reaction is detected by the blue color becoming bleached (due to the activity of methylene blue reductase). Bleaching may begin at the base of the test vial or just below the ball. Note that a residual blue ring is likely to remain around the ball, but this does not mean heterotrophs are absent.
        • A negative indication occurs when there is an absence of the blue color becoming bleached. This test is used to detect slimes, taste and odor, cloudiness and can also detect the amount of aerobic heterotrophic activity on hazardous waste sites.
      • BART™ Test for Iron-Related Bacteria (IRB), pk/9 (Product # 2432309), pk/27 (Product # 2432327) Method DOC316.53.01325
        • Iron-related bacterial populations oxidizing, reducing, and "accumulating" strains
      • BART™ Test for Nitrifying Bacteria, pk/7 (Product # 2619407) Method LIT8436 (Multiple test methods in document)
        • Ammonium oxidizing strains
        • Ammonium is a common product of the breakdown of organic nitrogen under anaerobic (oxygen-free) conditions. The nitrifying bacteria oxidize the ammonium to nitrate in soils and waters. This nitrate now becomes a hazard if it builds up in the water.
        • This test is a different form of BART™ test, in that it is fixed in length and involves the detection of nitrite as an indicator of the presence of nitrifying bacteria. Nitrate is not detected because the amounts can be transient where there is a considerable amount of denitrification.
        • A reaction cap is used to allow the detection of the nitrite after a five-day incubation period.
        • A positive reaction occurs when a coloring is produced in the reaction cap
        • A negative indication occurs when no coloring is produced in the reaction cap.
      • BART™ Test for Pool and Spa Bacteria, pk/9 (Product # 2478409) Method LIT8436 (Multiple test methods in document)
        • Problematic pool and spa bacterial; Pseudomonas strains
      • BART™ Test, Slime-Forming (SLYM) Bacteria, pk/9 (Product # 2432509), pk/27 (Product # 2432527) Method DOC316.53.01327
        • Aerobic/Anaerobic slime producing bacteria: Pseudomonads
        • A positive reaction involves a cloudy reaction in the inner test vial, often with thick gel-like rings around the ball.
        • A negative test remains clear.
      • BART™ Test, Sulfate-Reducing Bacteria (SRB), pk/9 (Product # 2432409), pk/27 (Product # 2432427) Method DOC316.53.01326
        • Anaerobic and aerobic hydrogen sulfide generating bacterial strains
        • Positive test occurs when there is a blackening, either in the base cone of the inner test vial (80% of the time) or around the ball (20% of the time).
        • A negative indication occurs when there is an absence of blackening in the base cone of the inner test vial or around the ball.
        • The culture medium is specific for the sulfate-reducing bacteria, such as Desulfovibrio and Desulfotomaculum. This is a more specific test and specifically relates to corrosion problems, taste & odor problems ("rotten egg" odors), and blackened waters. Slimes rich in SRB tend to also be black in color.
      • BART™ Test Combination Package: Iron-Related Bacteria (IRB), Sulfate-Reducing Bacteria (SRB), Slime-Forming (SLYM) Bacteria (3 each) (Product # 2434809)
    • Application Information
Application Problems associated with Bacterial growth Recommended BARTS™
Aquaculture Algal blooms can deplete Oxygen supplies and produce toxins ALG, NB
Cooling Tower and Heat Exchangers Spoilage of cooling tower waters. which necessitates costly dumping. Corrosion of equipment. IRB, SRB, SLYM, HAB, DN, NB
Well Drilling Drinking Water) Corrosion and plugging of wells. Masking of coliform bacteria. IRB, SRB, SLYM, HAB
Farms and Private Wells Corrosion and plugging of wells and distribution lines. Fluctuation of Iron and Magnesium levels. Nitrification levels of soil. IRB, SRB, SLYM, HAB, NB
Hazardous Waste Treatment Facilities Biofouling by anaerobic and aerobic bacteria, from extraction to treatment. IRB, SRB, SLYM, HAB
Municipal Water Treatment (DW & WW) Regrowth in distribution lines. Offensive taste and odor in potable water. Corrosion of pipes and fixtures. Biofouling and corrosion of wastewater treatment equipment. IRB, SRB, SLYM, FLOR, HAB, DN, NB
Petroleum: Oil Field Drilling and Refining Plugging of wells. Corrosion of pipes. IRB, SRB, SLYM
Pools and Spas Unsanitary conditions harbor disease POOL, HAB
Power Plant Utilities Biofouling. Corrosion and plugging of pipes, which reduces efficiency. IRB, SRB, SLYM, HAB
Process Water (Manufacturing) Corrosion and plugging of pipes. Which reduces efficiency. IRB, SRB, SLYM, HAB, DN, NB
Pulp and Paper Plants Plugging of pipes, reduced capacity, Speckled, poor-quality paper products. IRB, SRB, SLYM, DN, NB
Water Treatment Chemicals and Conditioning Biofouling and corrosion of cooling tower, boiler and home water systems. IRB, SRB, SLYM, HAB
  • Dip Slides & Paddle Testers
    • Concentration range: Paddle testers measure in the range from 100 organisms per mL to 100,000 organisms per mL by comparison to a colony density chart.
    • Media Packing
      • Paddle Tester, Total Aerobic Bacteria/Yeast and Mold, pk/10 (Product # 2610810) Method DOC316.53.01223
        • Tryptone glucose extract agar with Triphenyl Tetrazolium Chloride (TTC) for non-selective growth of total aerobic bacteria
          • TTC positive is red colonies
        • Modified sabouraud agar with Rose Bengal for selective isolation of yeasts and molds.
          • Rose Bengal positive is pink
          • Filamentous growth is positive for molds
      • Paddle Tester, Total Aerobic Bacteria/Total Coliforms, pk/10  (Product # 2610910) Method DOC316.53.01223
        • Tryptone glucose extract agar with Triphenyl Tetrazolium Chloride (TTC) for non-selective growth of total aerobic bacteria
          • TTC positive is red colonies
        • Violet red bile agar for enumerating total coliforms
          • Fermenting lactose coliforms produce pink colonies
          • Non-fermenter produce colorless gray colonies
      • Paddle Tester, Total Aerobic Bacteria/Disinfection Control, pk/10 (Product # 2619510) Method DOC316.53.01223
        • Tryptone glucose extract agar with Triphenyl Tetrazolium Chloride (TTC) for non-selective growth of total aerobic bacteria
          • TTC positive is red colonies
        • D/E neutralizing agar neutralizes several antiseptic and disinfectant chemicals.  Neutralization allows growth of bacteria held in bacteriostasis.

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