Microbiology Guide: Membrane Filtration (MF) Media

Document ID

Document ID TE8842

Version

Version 4.0

Status

Status Published

Published Date

Published Date 08/20/2019
Question
Microbiology Guide: Membrane Filtration (MF) Media
Summary
Microbiology Guide: Media and Equipment Selection; Membrane Filtration (MF) Media
Answer
From: Microbiology Guide: Media and Equipment Selection

Membrane Filtration (MF) Media Selection Summary

Drinking Water and Beverages
Organism USEPA- Accepted (Y/N) Presumptive Media Confirmation Media
Total Coliforms Y m-Endo Lauryl Tryptose (LT) Broth Tubes (Single-Strength) and Brilliant Green Bile Broth Tubes or Coli-Firm Kit
 
Total Coliforms N m-ColiBlue24® No Confirmation Necessary
Fecal Coliforms Y m-Endo EC Medium Tubes
E. Coli Y M-Endo EC Medium with MUG Tubes or Nutrient Agar with MUG Tubes
E. Coli N m-ColiBlue24® No Confirmation Necessary
Heterotrophic Bacteria N m-TGE or m-TGE with TTC Indicator No Confirmation Necessary
Yeast and Mold N m-Green YM No Confirmation Necessary

Wastewater and Recreational Water
Organism USEPA- Accepted (Y/N) Presumptive Media Confirmation Media
Fecal Coliforms Y m-FC or m-FC with Rosolic Acid No Confirmation Necessary
E. Coli Y m-TEC Agar Tubes Urea Substrate

Media With Confirmation
  • m-Endo Media, Hach method 8074, Presumptive for Total and Fecal Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • Adapted from Standard Methods (SM) 9222 B and 9221 B.
    • Packaging Options
      • m-Endo Broth, pk/20 2-mL PourRite™ Glass Ampules (Product # 2373520)
      • m-Endo Broth, Plastic Ampules, pk/50 (Product # 2373550)
      • m-Endo Broth, 100 mL glass bottle, 50 tests (Product # 2373542)
      • m-Endo Agar Plates, pk/15 (Product # 2811615)
    • Confirmation Necessary
      • Total Coliform: lauryl tryptose & brilliant green bile
      • Fecal Coliform: EC medium
      • E. coli Coliform: EC medium with MUG
 
  • m-ColiBlue24®, Hach method 10029, Simultaneous measurement for Total and E. coli Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • USEPA Approved
    • Packaging Options
      • m-ColiBlue24® Broth, Glass Ampules, pk/20 (Product # 2608420)
      • m-ColiBlue24® Broth, Plastic Ampules, pk/50 (Product # 2608450)
      • m-ColiBlue24® Broth, 100ml Bottle (50 test) (Product # 2608442)
      • m-ColiBlue24® Prepared Agar Plates, pk/15  (Product # 2805215)
    • Confirmation
      • Oxidase Reagent, 0.5 mL, pk/50 (Product # 2622500)
 
  • m-FC & m-FC/RA, Hach method 8074, Fecal Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • USEPA approved 9222 D
    • Packaging Options m-FC
      • m-FC 2 mL Glass Ampules, pk/20 (Product # 2373220)
      • m-FC media, 2 mLPlastic Ampules, pk/50 (Product # 2373250)
      • m-FC Agar Plates, pk/15  (Product # 2811515)
    • Packaging Options m-FC/RA
      • m-FC with Rosolic Acid Broth, Glass Ampules, pk/20 (Product # 2428520)
      • m-FC with Rosolic Acid, 2 mL Plastic Ampules, pk/50 (Product # 2428550)
    • Confirmation
      • Total Coliform: lauryl tryptose & brilliant green bile
      • Fecal Coliform: EC medium
      • E. coli Coliform: EC medium with MUG
   
  • Modified m-TEC, Hach method 8367, E. coli Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • USEPA accepted
    • Packaging Options
      • m-TEC, Modified, Prepared Agar Plates, pk/15 (Product # 2811815)
    • Confirmation not necessary
 
  • Heterotrophic Bacteria Media, Hach method 8242
    • For water and wastewater samples
    • m-HPC Broth, Plastic Ampules, pk/50 (Product # 2812450)
    • m-HPC Agar Plates,pk/15 (Product # 2811415)
    • m-TGE Broth, Plastic Ampules, pk/50 (Product # 2373850)
    • Tryptic Soy Broth Plastic Ampules, pk/50 (Product # 2812650)
    • Peel Plate® Heterotrophic Plate Count Media (Product # PP-HPC-50)
 
  • Enterococci Bacteria Media, Hach method 1600
    • For potable, nonpotable, recreation and wastewater samples
    • Packaging Options
      • m-EI Prepared Agar Plates, pk/15 (Product # 2811715)
 
  • Pseudomonas Bacteria Media, Hach method 8026
    • For potable, nonpotable, recreation and wastewater samples
    • Pseudomonas Broth, Plastic Ampules, pk/50 (Product # 2812250)
Media Decontamination
  • Recommendation:
    • Open each petri dish and pipet in 2-3 mL of Clorox (one can use a disposable plastic dropper).
    • Cap the petri dish and swirl the wet to whole membrane filter.  Let stand for 20 – 30 minutes.
    • After the waiting time, place all Cloroxed petri dishes in a Contaminated plastic bag for disposal.  Tie off or knot the bag.
    • Write "Cloroxed" on the Contamination bag.  Place into normal trash container. If using large plastic petri dishes, the process is the same except using 5 mL of Clorox to each plate.

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