Microbiology Guide: Membrane Filtration (MF) Media

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Microbiology Guide: Media and Equipment Selection; Membrane Filtration (MF) Media

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From: Microbiology Guide: Media and Equipment Selection

Membrane Filtration (MF) Media Selection Summary

Drinking Water and Beverages

Organism	USEPA- Accepted (Y/N)	Presumptive Media	Confirmation Media
Total Coliforms	Y	m-Endo	Lauryl Tryptose (LT) Broth Tubes (Single-Strength) and Brilliant Green Bile Broth Tubes or Coli-Firm Kit
Total Coliforms	Y	m-ColiBlue24®	No Confirmation Necessary
Fecal Coliforms	Y	m-Endo	EC Medium Tubes
E. Coli	Y	M-Endo	EC Medium with MUG Tubes or Nutrient Agar with MUG Tubes
E. Coli	Y	m-ColiBlue24®	No Confirmation Necessary
Heterotrophic Bacteria	N	m-TGE or m-TGE with TTC Indicator	No Confirmation Necessary
Yeast and Mold	N	m-Green YM	No Confirmation Necessary

Wastewater and Recreational Water

Media With Confirmation

m-Endo Media, Hach method 8074, Presumptive for Total and Fecal Coliform
- For potable, nonpotable, recreation and wastewater samples
  - Adapted from Standard Methods (SM) 9222 B and 9221 B.
- Packaging Options
  - m-Endo Broth, pk/20 2-mL PourRite™ Glass Ampules (Product # 2373520)
  - m-Endo Broth, Plastic Ampules, pk/50 (Product # 2373550)
  - m-Endo Broth, 100 mL glass bottle, 50 tests (Product # 2373542)
  - m-Endo Agar Plates, pk/15 (Product # 2811615)
- Confirmation Necessary
  - Total Coliform: lauryl tryptose & brilliant green bile
  - Fecal Coliform: EC medium
  - E. coli Coliform: EC medium with MUG

m-ColiBlue24®, Hach method 10029, Simultaneous measurement for Total and E. coli Coliform
- For potable, nonpotable, recreation and wastewater samples
  - USEPA Approved
- Packaging Options
  - m-ColiBlue24® Broth, Glass Ampules, pk/20 (Product # 2608420)
  - m-ColiBlue24® Broth, Plastic Ampules, pk/50 (Product # 2608450)
  - m-ColiBlue24® Broth, 100ml Bottle (50 test) (Product # 2608442)
  - m-ColiBlue24® Prepared Agar Plates, pk/15 (Product # 2805215)
- Confirmation
  - Oxidase Reagent, 0.5 mL, pk/50 (Product # 2622500)

m-FC & m-FC/RA, Hach method 8074, Fecal Coliform
- For potable, nonpotable, recreation and wastewater samples
  - USEPA approved 9222 D
- Packaging Options m-FC
  - m-FC 2 mL Glass Ampules, pk/20 (Product # 2373220)
  - m-FC media, 2 mLPlastic Ampules, pk/50 (Product # 2373250)
  - m-FC Agar Plates, pk/15 (Product # 2811515)
- Packaging Options m-FC/RA
  - m-FC with Rosolic Acid Broth, Glass Ampules, pk/20 (Product # 2428520)
  - m-FC with Rosolic Acid, 2 mL Plastic Ampules, pk/50 (Product # 2428550)
- Confirmation
  - Total Coliform: lauryl tryptose & brilliant green bile
  - Fecal Coliform: EC medium
  - E. coli Coliform: EC medium with MUG

Modified m-TEC, Hach method 8367, E. coli Coliform
- For potable, nonpotable, recreation and wastewater samples
  - USEPA accepted
- Packaging Options
  - m-TEC, Modified, Prepared Agar Plates, pk/15 (Product # 2811815)
- Confirmation not necessary

Heterotrophic Bacteria Media, Hach method 8242
- For water and wastewater samples
- m-HPC Broth, Plastic Ampules, pk/50 (Product # 2812450)
- m-HPC Agar Plates,pk/15 (Product # 2811415)
- m-TGE Broth, Plastic Ampules, pk/50 (Product # 2373850)
- Tryptic Soy Broth Plastic Ampules, pk/50 (Product # 2812650)
- Peel Plate® Heterotrophic Plate Count Media (Product # PP-HPC-50)

Enterococci Bacteria Media, Hach method 1600
- For potable, nonpotable, recreation and wastewater samples
- Packaging Options
  - m-EI Prepared Agar Plates, pk/15 (Product # 2811715)

Pseudomonas Bacteria Media, Hach method 8026
- For potable, nonpotable, recreation and wastewater samples
- Pseudomonas Broth, Plastic Ampules, pk/50 (Product # 2812250)

Media Decontamination

Recommendation:
- Open each petri dish and pipet in 2-3 mL of Clorox (one can use a disposable plastic dropper).
- Cap the petri dish and swirl the wet to whole membrane filter. Let stand for 20 – 30 minutes.
- After the waiting time, place all Cloroxed petri dishes in a Contaminated plastic bag for disposal. Tie off or knot the bag.
- Write "Cloroxed" on the Contamination bag. Place into normal trash container. If using large plastic petri dishes, the process is the same except using 5 mL of Clorox to each plate.

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