Question
Microbiology Guide: Membrane Filtration (MF) Media
Summary
Microbiology Guide: Media and Equipment Selection; Membrane Filtration (MF) Media
Answer
From: Microbiology Guide: Media and Equipment Selection
Membrane Filtration (MF) Media Selection Summary
*Please review the method document for specific EPA information. Additional information may also be located at the Hach EPA Compliant Methods website
Drinking Water and Beverages
Wastewater and Recreational Water
Media With Confirmation
Membrane Filtration (MF) Media Selection Summary
*Please review the method document for specific EPA information. Additional information may also be located at the Hach EPA Compliant Methods website
Drinking Water and Beverages
| Organism | USEPA- Accepted (Y/N) | Presumptive Media | Confirmation Media | Hach Method |
| Total Coliforms | Y | m-Endo | Lauryl Tryptose (LT) Broth Tubes (Single-Strength) and Brilliant Green Bile Broth Tubes or Coli-Firm Kit |
8074 m-Endo |
| Total Coliforms | Y | m-ColiBlue24® | No Confirmation Necessary | 10029 |
| Fecal Coliforms | Y | m-Endo | EC Medium Tubes | 8074 m-Endo |
| E. Coli | Y | m-Endo | EC Medium with MUG Tubes or Nutrient Agar with MUG Tubes | 8074 m-Endo |
| E. Coli | Y | m-ColiBlue24® | No Confirmation Necessary | 10029 |
| Heterotrophic Bacteria | N | m-TGE, m-TGE with TTC Indicator, m-HPC, m-TSB-USB | No Confirmation Necessary | 8282 - See below for links to method documents |
| Pseudomonas | N | Pseudomonas Broth Ampules | No Confirmation Necessary | 8026 |
| Enterococci (Coliforms) | Y | m-El Prepared Agar | No Confirmation Necessary | 1600 |
| Yeast and Mold | N | m-Green YM | No Confirmation Necessary | DOC316.53.01190 |
Wastewater and Recreational Water
| Organism | USEPA- Accepted (Y/N) | Presumptive Media | Confirmation Media | Hach Method |
| Fecal Coliforms | Y | m-FC or m-FC with Rosolic Acid | No Confirmation Necessary | 8074 m-FC |
| E. Coli | Y | m-TEC, modified | No Confirmation Necessary | 8367 |
| Pseudomonas | N | Pseudomonas Broth Ampules | No Confirmation Necessary | 8026 |
| Enterococci (Coliforms) | Y | m-El prepared Agar | No Confirmation Necessary | 1600 |
Media With Confirmation
-
m-Endo Media, Hach method 8074, Presumptive for Total and Fecal Coliform
- For potable, nonpotable, recreation and wastewater samples
- Adapted from Standard Methods (SM) 9222 B and 9221 B.
- Packaging Options
- m-Endo Broth, pk/20 2-mL PourRite™ Glass Ampules (Product # 2373520)
- m-Endo Broth, Plastic Ampules, pk/50 (Product # 2373550)
- m-Endo Broth, 100 mL glass bottle, 50 tests (Product # 2373542)
- m-Endo Agar Plates, pk/15 (Product # 2811615)
- Confirmation Necessary
- Total Coliform: lauryl tryptose broth tubes, 15/pk (Product # 2162315) & brilliant green bile tubes, 15/pk (Product # 32215)
- Fecal Coliform: EC medium tubes, 15/pk (Product # 1410415)
- E. coli Coliform: EC medium with MUG without Durham tubes, 15/pk (Product # 2471515)
- For potable, nonpotable, recreation and wastewater samples
-
m-ColiBlue24®, Hach method 10029, Simultaneous measurement for Total and E. coli Coliform
- For potable, nonpotable, recreation and wastewater samples
- USEPA Approved
- Packaging Options
- m-ColiBlue24® Broth, Glass Ampules, pk/20 (Product # 2608420)
- m-ColiBlue24® Broth, Plastic Ampules, pk/50 (Product # 2608450)
- m-ColiBlue24® Broth, 100ml Bottle (50 test) (Product # 2608442)
- m-ColiBlue24® Prepared Agar Plates, pk/15 (Product # 2805215)
- Confirmation is not necessary, but if the oxidase methods need to be run the following item needs to be purchased.
- Oxidase Reagent, 0.5 mL, pk/50 (Product # 2622500)
- For potable, nonpotable, recreation and wastewater samples
-
m-FC & m-FC/RA, Hach method 8074, Fecal Coliform
- For potable, nonpotable, recreation and wastewater samples
- USEPA approved 9222 D
- Packaging Options m-FC
- m-FC 2 mL Glass Ampules, pk/20 (Product # 2373220)
- m-FC media, 2 mLPlastic Ampules, pk/50 (Product # 2373250)
- m-FC Agar Plates, pk/15 (Product # 2811515)
- Packaging Options m-FC/RA
- m-FC with Rosolic Acid Broth, Glass Ampules, pk/20 (Product # 2428520)
- m-FC with Rosolic Acid, 2 mL Plastic Ampules, pk/50 (Product # 2428550)
- Confirmation
- Total Coliform: lauryl tryptose broth tubes, 15/pk (Product # 2162315) & brilliant green bile tubes, 15/pk (Product # 32215)
- Fecal Coliform: EC medium tubes, 15/pk (Product # 1410415)
- E. coli Coliform: EC medium with MUG without Durham tubes, 15/pk (Product # 2471515)
- For potable, nonpotable, recreation and wastewater samples
-
Modified m-TEC, Hach method 8367, E. coli Coliform
- For potable, nonpotable, recreation and wastewater samples
- USEPA accepted
- Packaging Options
- m-TEC, Modified, Prepared Agar Plates, pk/15 (Product # 2811815)
- Confirmation not necessary
- For potable, nonpotable, recreation and wastewater samples
-
Heterotrophic Bacteria Media, Hach method 8242
- For water and wastewater samples
- Mutliple versions of method 8282 are available. The following links will pull up each different method document:
- m-TGE Broth, Membrane Filtration
- m-TGE Broth, Membrane Filtration, with TTC Indicator
- m-TGE Broth, Pour Plate
- m-TGE Broth, Pour Plate, with TTC indicator
- m-HPC, Membrane Filtration
- m-HPC, Pour Plate
- m-TSB-USP, Membrane Filtration
-
m-TSB-USB, Pour Plate
- m-HPC Broth, Plastic Ampules, pk/50 (Product # 2812450)
- m-HPC Agar Plates,pk/15 (Product # 2811415)
- m-HPC Agar Plates, pk/15 x 6 (Product # 2811415-90)
- m-TGE Broth, Plastic Ampules, pk/50 (Product # 2373850)
- m-TGE/TTC Broth, Plastic Ampules, pk/50 (Product # 2428450)
- Tryptic Soy Broth Plastic Ampules, pk/50 (Product # 2812650)
-
Enterococci Bacteria Media, Hach method 1600
- For potable, nonpotable, recreation and wastewater samples
- Packaging Options
- m-EI Prepared Agar Plates, pk/15 (Product # 2811715)
-
Pseudomonas Bacteria Media, Hach method 8026
- For potable, nonpotable, recreation and wastewater samples
- Pseudomonas Broth, Plastic Ampules, pk/50 (Product # 2812250)
Media Decontamination
-
Recommendation:
- Open each petri dish and pipet in 2-3 mL of Clorox (one can use a disposable plastic dropper).
- Cap the petri dish and swirl the wet to whole membrane filter. Let stand for 20 – 30 minutes.
- After the waiting time, place all Cloroxed petri dishes in a Contaminated plastic bag for disposal. Tie off or knot the bag.
- Write "Cloroxed" on the Contamination bag. Place into normal trash container. If using large plastic petri dishes, the process is the same except using 5 mL of Clorox to each plate.