Microbiology Guide: Membrane Filtration (MF) Media

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Document ID TE8842

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Published Date 01/13/2022
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Microbiology Guide: Membrane Filtration (MF) Media
Summary
Microbiology Guide: Media and Equipment Selection; Membrane Filtration (MF) Media
Answer
From: Microbiology Guide: Media and Equipment Selection

Membrane Filtration (MF) Media Selection Summary
*Please review the method document for specific EPA information. Additional information may also be located at the Hach EPA Compliant Methods website

Drinking Water and Beverages
Organism USEPA- Accepted (Y/N) Presumptive Media Confirmation Media Hach Method
Total Coliforms Y m-Endo Lauryl Tryptose (LT) Broth Tubes (Single-Strength) and Brilliant Green Bile Broth Tubes or Coli-Firm Kit
 
8074 m-Endo
Total Coliforms Y m-ColiBlue24® No Confirmation Necessary 10029
Fecal Coliforms Y m-Endo EC Medium Tubes 8074 m-Endo
E. Coli Y m-Endo EC Medium with MUG Tubes or Nutrient Agar with MUG Tubes 8074 m-Endo
E. Coli Y m-ColiBlue24® No Confirmation Necessary 10029
Heterotrophic Bacteria N m-TGE,  m-TGE with TTC Indicator, m-HPC, m-TSB-USB No Confirmation Necessary 8282 - See below for links to method documents
Pseudomonas N Pseudomonas Broth Ampules No Confirmation Necessary 8026
Enterococci (Coliforms) Y m-El Prepared Agar No Confirmation Necessary 1600
Yeast and Mold N m-Green YM No Confirmation Necessary DOC316.53.01190

Wastewater and Recreational Water
Organism USEPA- Accepted (Y/N) Presumptive Media Confirmation Media Hach Method
Fecal Coliforms Y m-FC or m-FC with Rosolic Acid No Confirmation Necessary 8074 m-FC
E. Coli Y m-TEC, modified No Confirmation Necessary 8367
PseudomonasPseudomonas N Pseudomonas Broth Ampules No Confirmation Necessary 8026
Enterococci (Coliforms) Y m-El prepared Agar No Confirmation Necessary 1600

Media With Confirmation
  • m-Endo Media, Hach method 8074, Presumptive for Total and Fecal Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • Adapted from Standard Methods (SM) 9222 B and 9221 B.
    • Packaging Options
      • m-Endo Broth, pk/20 2-mL PourRite™ Glass Ampules (Product # 2373520)
      • m-Endo Broth, Plastic Ampules, pk/50 (Product # 2373550)
      • m-Endo Broth, 100 mL glass bottle, 50 tests (Product # 2373542)
      • m-Endo Agar Plates, pk/15 (Product # 2811615)
    • Confirmation Necessary
      • Total Coliform: lauryl tryptose broth tubes, 15/pk (Product # 2162315) & brilliant green bile tubes, 15/pk (Product # 32215)
      • Fecal Coliform: EC medium tubes, 15/pk (Product # 1410415)
      • E. coli Coliform: EC medium with MUG without Durham tubes, 15/pk (Product # 2471515)
 
  • m-ColiBlue24®, Hach method 10029, Simultaneous measurement for Total and E. coli Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • USEPA Approved
    • Packaging Options
      • m-ColiBlue24® Broth, Glass Ampules, pk/20 (Product # 2608420)
      • m-ColiBlue24® Broth, Plastic Ampules, pk/50 (Product # 2608450)
      • m-ColiBlue24® Broth, 100ml Bottle (50 test) (Product # 2608442)
      • m-ColiBlue24® Prepared Agar Plates, pk/15  (Product # 2805215)
    • Confirmation is not necessary, but if the oxidase methods need to be run the following item needs to be purchased.
      • Oxidase Reagent, 0.5 mL, pk/50 (Product # 2622500)
 
  • m-FC & m-FC/RA, Hach method 8074, Fecal Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • USEPA approved 9222 D
    • Packaging Options m-FC
      • m-FC 2 mL Glass Ampules, pk/20 (Product # 2373220)
      • m-FC media, 2 mLPlastic Ampules, pk/50 (Product # 2373250)
      • m-FC Agar Plates, pk/15  (Product # 2811515)
    • Packaging Options m-FC/RA
      • m-FC with Rosolic Acid Broth, Glass Ampules, pk/20 (Product # 2428520)
      • m-FC with Rosolic Acid, 2 mL Plastic Ampules, pk/50 (Product # 2428550)
    • Confirmation
      • Total Coliform: lauryl tryptose broth tubes, 15/pk (Product # 2162315) & brilliant green bile tubes, 15/pk (Product # 32215)
      • Fecal Coliform: EC medium tubes, 15/pk (Product # 1410415)
      • E. coli Coliform: EC medium with MUG without Durham tubes, 15/pk (Product # 2471515)
   
  • Modified m-TEC, Hach method 8367, E. coli Coliform
    • For potable, nonpotable, recreation and wastewater samples
      • USEPA accepted
    • Packaging Options
      • m-TEC, Modified, Prepared Agar Plates, pk/15 (Product # 2811815)
    • Confirmation not necessary
   
 
  • Enterococci Bacteria Media, Hach method 1600
    • For potable, nonpotable, recreation and wastewater samples
    • Packaging Options
      • m-EI Prepared Agar Plates, pk/15 (Product # 2811715)
 
  • Pseudomonas Bacteria Media, Hach method 8026
    • For potable, nonpotable, recreation and wastewater samples
    • Pseudomonas Broth, Plastic Ampules, pk/50 (Product # 2812250)
Media Decontamination
  • Recommendation:
    • Open each petri dish and pipet in 2-3 mL of Clorox (one can use a disposable plastic dropper).
    • Cap the petri dish and swirl the wet to whole membrane filter.  Let stand for 20 – 30 minutes.
    • After the waiting time, place all Cloroxed petri dishes in a Contaminated plastic bag for disposal.  Tie off or knot the bag.
    • Write "Cloroxed" on the Contamination bag.  Place into normal trash container. If using large plastic petri dishes, the process is the same except using 5 mL of Clorox to each plate.

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