Can a calibration in the Lachat Omnion software be moved to another run or reused?

Document ID

Document ID TE8484

Published Date

Published Date 09/06/2019
Question
Can a calibration in the Lachat Omnion software be moved to another run or reused?
Summary
Moving or reusing a calibration
Answer
It is possible to reuse a calibration and to replace the calibration used to analyze a sample run. There are two methods to reuse a calibration. Method 2 can also be used to apply a calibration to a previous run, allowing one to reprocess samples and QC against a different calibration curve.

Method 1 - Editing the run worksheet.
 
  • An existing run file which includes a calibration can be used for samples which have not yet been run.
  • The worksheet can be edited to define the samples and QC standards to be run. The calibration standards must to be removed (deleted) from the Run worksheet. It is important that no calibration standards exist within the run.
  • The calibration visible in the Calibration Results window is the calibration that will be used.
  • The Calibration Results can be viewed by selecting the Calibration Results button from the left-hand side of the channel data display.
  • As the samples are processed, data will be generated in a new file and the calibration visible in the  Calibration Results window will be used.

Method 2 - Copying the calibration from one run file to another.
 
  • Omnion allows four runs to be opened at any time.   

     
    The first .omn file opened will be opened in Run1. The second .omn file opened will be opened in Run2, etc. Switching between the runs can be accomplished by selecting the Run button for the file they to be viewed/edited. The run currently selected will show up bold and underlined.
     
  • Two runs will need to be opened to copy the calibration. In this example Run 1 will contain the calibration to be copied and Run 2 will contain the run file the calibration curve is to be copied to.
  • If the Analyte tab within the Run Properties window of Run 1 is selected, properties of the Analyte can be viewed. Selecting the Analyte from the list on the left-hand side will reveal a drop-down menu. Choosing copy, will copy all the properties of the analyte, one of which is the calibration.     
  • Once the analyte has been copied it can be moved to a different run. Selecting Run2, then navigating to the Analytes tab of the Run Properties window, will allow access to the analyte in Run2. Selecting the Analyte from the list on the left-hand side will again reveal a drop-down menu. Choosing Paste, will Paste all the properties of the analyte including the calibration.
  • If the purpose of moving the calibration is to apply it to samples that have already been run, an additional step is required after the calibration curve is copied and pasted. Right-clicking within the channel data display (the window peaks are visible in) will reveal a drop down menu. Choosing "Rerun Peak Detection Ignoring Calibration Standards" will reprocess the samples and ignore the original calibration.



For either of these methods, one can verify the calibration curve being used by selecting the Calibration Results button from the left-hand side of the channel data display.

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