What could cause very small peaks when running samples and standards on a Lachat Flow Injection Analysis Method?
Small peaks when running Lachat Flow Injection Analysis (FIA) methods.
If peaks are significantly smaller than the support data on a Lachat FIA method, there are several things that could be at fault:
- Verify the valve timing. Run dye as a sample and verify the sample loop is being filled properly and injected into the manifold.
- There could be a valve issue. Try using a different injection valve.
- Verify the reagents are fresh and free from contamination. Old source chemicals can also be a concern even if the reagent was freshly prepared.
- Verify the pH of the reagents, samples and standards against the method.
- Check the standard source and preparation.
- Verify the correct sample loop for the method is used.
- Verify the correct pump tubes are being used. Check both the size and the material.
- Verify the correct detector filter is used.
- Try using a different detector to verify the detector is functioning properly.
- Check the baseline voltage.
- Check the pump to verify it is working at the correct speed.