Can the standard IntelliCAL™ pH probes be used with TRIS buffers?

Document ID

Document ID TE3092

Published Date

Published Date 07/12/2018
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Question
Can the standard IntelliCAL™ pH probes be used with TRIS buffers?
Summary
IntelliCAL™ pH probe TRIS compatibility
Answer
No, both the gel filled (standard and rugged) and refillable IntelliCAL™ probes have a silver/silver chloride reference. These would not be TRIS compatibile and will result in inaccurate pH measurement due to precipitation of the silver. A Red Rod IntelliCAL™ probe would be required for use in TRIS samples.
 

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