How should sample containers be prepared for microbiological testing?

Document ID

Document ID TE2846

Published Date

Published Date 07/31/2019
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Question
How should sample containers be prepared for microbiological testing?
Summary
Microbiology sample container preparation
Answer
To collect samples, use any of the following: sterilized plastic bags, sterilized disposable bottles, autoclavable glass or plastic bottles.

Presterilized plastic bags and bottles are available with or without dechlorinating reagent.

Dechlorinating reagent should be used with potable or chlorinated water samples (though it can be used optionally with nonchlorinated samples).

Glass or plastic bottles may be used instead of sterilized bags or disposable bottles if they are prepared as follows:
  • Wash in hot water and detergent.
  • Thoroughly rinse with hot tap water, followed by a distilled water rinse to remove all detergent.
  • If dechlorinating reagent is needed, add the contents of one Dechlorinating Reagent Powder Pillow (Product # 1436369) for each 125-mL of container volume.
  • Steam sterilize glass and autoclavable plastic containers at 121 °C (250°F) for 15 minutes. Glass sample containers may be sterilized by hot air at 170 °C (338 °F) for one hour.
  • Store sterile containers, tightly capped, in a clean environment until needed.

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