What is the purpose of (presence/absence) P/A Broth and how is it used?

Document ID

Document ID TE1005

Published Date

Published Date 04/12/2018
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Question
What is the purpose of (presence/absence) P/A Broth and how is it used?
Summary
P/A Broth is used to screen drinking water for Total coliforms.
Answer
P/A Broth is ideal for screening drinking water samples for total coliforms. The method is a simple modification of the multiple-tube method. It uses lactose and lauryl tryptose broths with bromcresol puple, which detects acidity formed during lactose fermentation by the bacteria. Simply combine 100 mL of sample and P/A broth, indubate for 24 hours and check for a color change. A yellow color indicates the presence of total coliforms.

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