In microbiological testing, are there any special steps to take if the sample is less than 20 mL (diluted or undiluted)?

Document ID

Document ID TE993

Version

Version 3.0

Status

Status Published

Published Date

Published Date 04/24/2018
Question
In microbiological testing, are there any special steps to take if the sample is less than 20 mL (diluted or undiluted)?
Summary
Microbiological testing sample volume
Answer
In microbiological testing, if the sample is less than 20 mL (diluted or undiluted), add 10 mL of sterile dilution water to the filter funnel before applying the vacuum. This aids in distributing the bacteria evenly across the entire filter surface.

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