Can the m-ColiBlue24® method be used to test for R. Terrigena?

Document ID

Document ID TE870

Version

Version 3.0

Status

Status Published

Published Date

Published Date 10/09/2018
Question
Can the m-ColiBlue24® method be used to test for R. Terrigena?
Summary
Hach method for Raoultella Terrigena Bacteria
Answer
Raoultella terrigena (also known as Klebsiella terrigena) is a rarely encountered gram-negative bacteria and mainly reported as aquatic and soil organism. Raoultella terrigena ATCC 33257, a representative of the coliform group, is commonly used as a challenge organism in water purifier efficacy testing. In addition to being time consuming, traditional culturing techniques and metabolic identification systems (including automated systems) also fail to accurately differentiate this organism from its closely related neighbors belonging to the Enterobacteriaceae group. Molecular-based techniques, such as real-time quantitative polymerase chain reaction (qPCR) and enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting, are preferred methods of detection because of their accuracy, reproducibility, specificity, and sensitivity, along with shorter turnaround time. Enterobacter, Klebsiella, and Citrobacter are expected to produce a red colony on m-ColiBlue24®; however, this specific species has not been tested and may not work accurately as compared to other testing methods.

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