What is the best dilution water to use for diluting microbiological samples?

Document ID

Document ID TE346

Published Date

Published Date 05/02/2019
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Question
What is the best dilution water to use for diluting microbiological samples?
Summary
Water for diluting microbiological samples
Answer
Use water that is buffered to a neutral pH and sterilized for microbiological testing. Hach dilution water containing magnesium chloride and potassium phosphate is recommended for dilution of most nonpotable and wastewater samples. Magnesium Chloride/Potassium Phosphate Buffer, 99 mL, pk/25 (Product # 1430598) can be used.
-Add 11 mL of sample to a bottle and mix. This will result in a 10-fold dilution. See the Hach procedure for the test that you are using for more specific instructions.


This can also be prepared from Buffered Dilution Water Pillows, pk/25 (Product # 2143166).
This item contains 25 magnesium chloride pillows (large) and 25 potassium phosphate pillows (small).
-Add one pillow of magnesium chloride and one pillow of potassium phosphate to one liter of distilled water and autoclave for 15 minutes.


-Use Butterfield's Buffered Phosphate (Product # 2319109, 2319125, 2319145, or 2319110) or Dilution Water Concentrate Pillows, pk/25 (Product # 2323668) when testing food samples, or whenever the procedure specifies this water for dilution.
 

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