Does CuVer® react with chelated copper and is it possible to determine what portion of the result is free and chelated copper?

Document ID

Document ID TE261

Published Date

Published Date 05/21/2019
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Question
Does CuVer® react with chelated copper and is it possible to determine what portion of the result is free and chelated copper?
Summary
Determining the concentration of free and chelated copper
Answer
CuVer® does not recover fully chelated copper. If there are other metals in the sample, or anything that might strongly chelate the copper, it will interfere with the CuVer® . Digestion of the sample either using the EPA approved mild or vigorous digestion is recommended.

To differentiate between free and copper that is complexed to EDTA or other complexing agents:

-Use the Copper Reagent Set, Free and Total (Product # 2439200; includes the Free Copper Reagent Powder Pillows, 25 mL, pk/100 Product # 2118669 and Hydrosulfite Reagent Powder Pillows, 25 mL, pk/100 Product # 2118869).
-Use a 25-mL sample with 25ml of sample and a Free Copper reagent powder pillow instead of CuVer® 1. This result will be in mg/L free copper.
-Add a Hydrosulfite Reagent powder pillow to the same sample, wait two minutes, and re-read result. This result will be in mg/L total dissolved copper.
-To determine the mg/L of complexed copper, subtract the mg/L free copper from the mg/L total dissolved copper.
 

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