Are instructions available for using PolySeed®?

Document ID

Document ID TE181

Published Date

Published Date 08/27/2020
Are instructions available for using PolySeed®?
Polyseed® Instructions
Polyseed® is a blend of microbial cultures packaged in capsules for analysis of Biochemical Oxygen Demand (BOD).  Polyseed® is designed to provide a uniform standard for the  degradation of both industrial and municipal wastes in BOD analysis.

1. Prepare the seed by adding one PolySeed® capsule to 500 mL of dilution water, stir gently, and aerate for one hour.
2. The dilution water must contain a nutrient buffer pillow as described in the test procedure.

Seed Control:
The seed itself will consume some Dissolved Oxygen (DO) that must be measured and used in calculating a seed correction factor.
1. Run the seed as a normal sample. The oxygen depletion should be at least 2 mg/L DO.
2. Use the depletion, as well as ratio of volume of seed in sample to seed in the control, to correct for the oxygen demand from the seed.

1. For the dilution method, add 4 mL (amount varies) of seed directly to each BOD bottle when measuring samples or standards.
* If the seed strength is unknown, use 20 mL when using the BODTrak™ II.

This is described in more detail in the test procedure for each method. An instruction document from the manufacturer is also attached to this article.

Additional resources can be found at


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