How should the glassware used for the BOD dilution method be cleaned?

Document ID

Document ID TE176

Published Date

Published Date 08/20/2019
Question
How should the glassware used for the BOD dilution method be cleaned?
Summary
Glassware preparation for BOD method
Answer
When testing Biochemical Oxygen Demand (BOD), very clean glassware is required to avoid any contamination.

To clean BOD bottles:

1. Use a 20 to 50% sulfuric acid solution (approximately 5 to 14 N).
2. Pour several mL of the acid into each bottle, cap, and invert to coat the inner surfaces of the bottles.
3. Rinse thoroughly with distilled water.

Use this acid rinse in the dilution bottle, fittings, tubing, glassware, and in any other labware that comes into contact with the sample.

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