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Are there instructions for using the BOD Standard Solution, 300 mg/L, pk/16 - 10-mL Voluette® Ampules?

Document ID

Document ID TE167

Published Date

Published Date 02/08/2021
Question
Are there instructions for using the BOD Standard Solution, 300 mg/L, pk/16 - 10-mL Voluette® Ampules?
Summary
BOD Standard Solution, 300 mg/L, pk/16 - 10-mL Voluette® Ampules
The BOD Standard Solution, 300 mg/L, pk/16 - 10-mL Voluette® Ampules  (Product # 1486510) is used to validate technique. It is composed of 300 mg each, of Glucose and Glutamic Acid (GGA). This standard is therefore double the concentration of the GGA standard specified in Standard Methods for the Examination of Water and Wastewater, method 5210.

There are two methods for analyzing the standard:

The first method is called the Graphical Method:

•  Add 1, 2, 3, and 4 mL of the standard to separate BOD bottles.
•  The bottles are then filled with seeded dilution water and incubated at 20 °C for 5 days.
•  The amount of dissolved oxygen remaining after 5 days is plotted against the volume of the standard used and a line of best fit (first order) is drawn through the points.
•  The slope of the line is determined, and this value is multiplied by the volume of the BOD bottle to determine BOD value of the standard.
•  The calculated value is then divided by 2 (since the GGA value of the standard is double that of Standard Methods) and the end result should be 198 +/- 30.5 mg/L.

This procedure is also described in the attached pdf file.

An alternate method (based on Standard Methods) is described below:

•  Add 3 mL of the standard to a BOD bottle (since the GGA value of the standard is double that of Standard Methods, 3 mL, instead of 6 mL of standard is used).
•  Fill the bottle with seeded dilution water and incubate at 20 °C for 5 days.
•  Perform the same calculations on the standard (calculate using 6 mL) , as on samples to determine the BOD value of the standard.
•  The BOD should be 198 +/- 30.5 mg/L.
•  It is recommended this analysis be run in triplicate.
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